

They put the tardigrades onto surfaces cooled to below -226 degrees Fahrenheit (-143 degrees Celsius), and covered the tiny creatures in anisole, an anise-scented organic compound.
If it’s not enough that they froze them, then they covered them in licorice flavor and tattooed them with it. I literally didn’t realize you could torture something so.
The stress is from cooling the filament and then winding it around the spool. Lots of reasons why the plastic would weaken that stress might suddenly give way, but most of them involve time. If it wasn’t completely cooled internally before being spooled, that would dramatically increase the stress (think Prince Rupert’s drop).